Title: Biosynthesis, Characterization and Optimization of Auxins by Piriformospora indica: It’s Impact on Growth of Solanum melongena L.Cultivars
Authors: Swetha, S; Padmavathi, T
Abstract: The
Present study summarizes the biosynthesis, characterization and optimization of
auxins by Piriformospora indica and
its effect on the growth of Solanum
melongena L. cultivars. The biosynthesis of auxins was greatly influenced
by the addition of tryptophan. Based on the data obtained from traditional one factor at a time method,
optimization was carried out using the response surface methodology taking into
account three significant variables viz. tryptophan concentration, pH and trace
element solution. The optimized value of Indole 3 acetic acid and related
indolic metabolites was observed to be 7.39 mg mL-1 with 0.2 g of
tryptophan, pH 8.5 and 200
l trace element solution. The Presence of auxins
was, confirmed by UV visible spectroscopy, Fourier transform infrared
spectroscopy and mass spectroscopy. Mass spectroscopy analysis confirmed the
presence of Indole 3 acetic acid and related indolic metabolites. Pot studies
depicted a significant effect of auxins on both the cultivars of Solanum melongena L.
Page(s): 45-50
